#Dog snapper manual

Parameters were measured prior to and immediately after transportation, and at 24 and 96 hours recovery after transportation. The tested densities were 65, 80, 95, 110 and 125 g L-1 for fingerlings, and 50, 80, 110, 140 and 170 g L-1 for juveniles (three replicates each).

We assessed the effect of stocking density on physiological parameters (blood lactate, glucose, cortisol, hematocrit), water quality (temperature, dissolved oxygen, pH, unionized ammonia, carbon dioxide), and survival during the transportation of fingerling (24.5 В± 4.7 g) and juvenile (615.8 В± 122.2 g) pirarucu (Arapaima gigas) for six hours in plastic bags.

We develop recommendations that can provide practical guidance for fish biologists to help to obtain reliable and reproducible results. The aim of this work was to review the procedures used in fish hematology by various authors. The results of hematological analysis may be affected by various preanalytical and analytical factors.

#Dog snapper manual

Manual hematological methods that are most often used in fish hematology do not require highly specialized laboratory equipment, however automatic analysers adapted for use with fish blood are also in use. The use of hematological techniques is arduous and requires experience but high amount of information and relatively low cost are considerable advantages. Peripheral blood tests involve measurement of various red and white blood cells indices and are often supplemented by biochemical analyses. Hematological parameters were proved to be highly sensitive to various environmental factors including nutrition, water quality, stress or pathogens. Hematological analyses are commonly used to evaluate fish health and welfare in aquaculture, veterinary practice and scientific research. This study is the first to report stress response measurements of hematological and biochemical indicators in dog snapper, representing an important basis for the planning of future experiments involving the transport and handling of this fish species. Biochemical parameters revealed the effects of stress during transport, and after 24 h, glucose levels and hematological parameters (hemoglobin, erythrocytes, leukocytes, neutrophils and MCH) showed a tendency to return to control levels.

Biochemical and hematological results demonstrated the inefficiency of benzocaine as a stress reliever during handling and transport. Immediately after transport and after 24 h, blood was collected from the fish that underwent each treatment (with anesthetic and without anesthetic). Blood samples were collected prior to transport (control), and fish were placed in two transport boxes, one with anesthetic and one without anesthetic. Twenty-five wild dog snapper specimens were used in the experiment (220 В± 68 g and 24.5 В± 2.5 cm total length). The objective of this study was to evaluate stress responses in dog snapper (Lutjanus jocu) during transport by evaluating their hematological and biochemical responses.